منابع مشابه
Statistical mechanical simulation of polymeric DNA melting with MELTSIM
MOTIVATION MELTSIM is a windows-based statistical mechanical program for simulating melting curves of DNAs of known sequence and genomic dimensions under different conditions of ionic strength with great accuracy. The program is useful for mapping variations of base compositions of sequences, conducting studies of denaturation, establishing appropriate conditions for hybridization and renaturat...
متن کاملStatistical mechanics of base stacking and pairing in DNA melting.
We propose a statistical mechanics model for DNA melting in which base stacking and pairing are explicitly introduced as distinct degrees of freedom. Unlike previous approaches, this model describes thermal denaturation of DNA secondary structure in the whole experimentally accessible temperature range. Base pairing is described through a zipper model, base stacking through an Ising model. We p...
متن کاملA model for melting of confined DNA
When DNA molecules are heated they denature. This occurs locally so that loops of molten single DNA strands form, connected by intact double-stranded DNA pieces. The properties of this ‘melting’ transition have been intensively investigated. Recently there has been a surge of interest in this question, caused by experiments determining the properties of partially bound DNA confined to nanochann...
متن کاملMaking DNA melting useful.
Featured Article: Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 2003;49:853– 60. DNA melting (the denaturation of DNA by heat) is a fundamental property of DNA that can be used as an analytical tool. Classic studies of DNA melting monitored the absorbance near 260 nm as the temperature was increased and...
متن کاملRigidity of melting DNA.
The temperature dependence of DNA flexibility is studied in the presence of stretching and unzipping forces. Two classes of models are considered. In one case the origin of elasticity is entropic due to the polymeric correlations, and in the other the double-stranded DNA is taken to have an intrinsic rigidity for bending. In both cases single strands are completely flexible. The change in the e...
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ژورنال
عنوان ژورنال: Eclética Química
سال: 2001
ISSN: 0100-4670
DOI: 10.1590/s0100-46702001000100006